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The Liquid Injection System (LIS)

 
LIS is the first easy to use technology for automated feeding of liquids in shake flasks. LIS consists of three components: a sterile single-use cartridge, the LIS Drive and the LIS Software. Fill the cartridge with up to 25ml of any type of liquid, program the LIS Drive manually or wirelessly with the LIS Software and install the cartridge and drive on the flask. LIS will now run your chosen feeding profile and allows you to unlock completely new experimental possibilities in shake flasks.

Key Facts

Compatible with various substances

Sugars (e.g. 50 % glucose), alcohols (e.g. methanol), glycerol, acids, bases and more

Choose from different feeding profiles

Single-shot, multi-shot, constant feeding, exponential feeding and more

Easy to install and use

Fill the cartridge, program the LIS Drive and start feeding your culture

For a broad range of applications

Fed-batch, pH regulation, induction with IPTG, phage assays, toxicity tests and more

A flexible solution for your bioprocess

For all shakers, shake flask sizes and all typical shaking conditions

 

Exemplary Applications

 






Get LIS!

Do you want to perform feeding experiments in shake flasks?

LIS Drive

What is the LIS Drive?

The LIS Drive is a programmable miniature pump that controls when and how much liquid is fed from the LIS cartridge into the flask. You can either program the feeding profile in the LIS Drive manually using the wheel on top of the drive or wirelessly by using the LIS Software.

Do I have to clean the LIS Drive?

No, the LIS Drive is never in touch with the actual liquid but separated from it by a sterile filter. Thus, the LIS Drive can simply be reused after every experiment. Since the LIS Drive contains sensitive electronics, it can not be autoclaved

What are the minimal and maximal dispensing volumes?

The minimal dispensing volume is a single drop. Depending on the type of liquid, concentration and temperature this volume can vary but is typically between 40-50µl. The maximal dispensing volume is limited by the maximal flow rate of the LIS Drive, which is 1000µl per minute.

Under which shaking conditions can I use LIS?

The following matrix provides an overview on the maximal shaking speeds that can be used at 10 % filling volume of the flask together with LIS. Please note that these speeds are only valid for relatively new clamps and sticky stuff. Please test individually at which speed your flasks can be shaken safely together with LIS. If you intend to use LIS at shaking speeds higher than the ones listed, please contact us directly. We can supply special clamps that allow for high speed applications.

Which feeding profiles are available?

Currently the following profiles are available. Additional feeding profiles and a feeding profile generator are available in our LIS Software.

LIS Cartridge

What is the LIS Cartridge?

The LIS Cartridge is a single use container with the shape of a lid for shake flasks. It fits on all shake flasks that are normally sealed with a metal cap (straight neck shake flasks without a beaded rim and 38mm neck diameter). The cartridge comes sterile packed and is for one time use only. It can be filled with up to 25ml of any type of liquid and acts as a reservoir during the feeding experiment.

How much liquid can be filled into the LIS Cartridge?

Currently, LIS Cartridges can be filled with up to 25ml of any liquid. If you want to feed more than 25ml, you can simply switch cartridges during the experiment and replace the empty one with a newly prepared one.

Can I reuse LIS Cartridges?

No, the LIS Cartridge is a single use product that comes sterile. Sterility is not given after experimental usage. Additionally, the cartridge must be sealed air tight after filling with liquid for precise feeding in combination with the LIS Drive. aquila biolabs can only guarantee this proper fit (snap fit for air tightness between the cartridge and the LID for the first time use.

Does the LIS cartridge influence the oxygen transfer rate?

The LIS cartridge has a similar design to a standard metal cap for shake flasks. It does not fully close the shake flask but has spacers that allow air to flow into the flask by convection. Hence, the LIS cartridge and a standard metal cap seal lead to comparable oxygen transfer rates in your shake flask

Which liquids can I fill into the LIS Cartridge?

The table below provides an overview of successfully tested substances. If you are interested in a certain liquid and concentration that is not listed here, please contact us directly and we will inform or test for you if you can use your intended liquid.

 

 

 

 

 

 

 

 

 

How to set up LIS

How do I set up LIS?

In order to prepare and install LIS correctly, please take a look at our LIS – Quick Start Guide or contact us directly.

Coming Soon!

Informational Material

Papers

Coming Soon!