The Liquid Injection System (LIS)
Do you want to perform feeding experiments in shake flasks?
Discover Liquid Injection System (LIS)
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Key Facts
Wireless control and monitoring
Control and monitor your feeding experiments wirelessly with the LIS Software
Compatible with various substances
Sugars (e.g. 50 % glucose), alcohols (e.g. 75% methanol), glycerol (up to 75%), acids, bases, inductors (e.g. IPTG), antifoam and more
Create individual feeding profiles
Single/multi shot, constant/exponential feeding and many more
Easy to install and use
Fill the sterile cartridge, program the LIS drive and start feeding your culture
For a broad range of applications
Fed-batch, gene induction, pH regulation, phage assays, toxicity tests and more
Exemplary Applications
What is the LIS Drive?
The Drive is a programmable miniature pump that controls when and how much liquid is fed from the cartridge into the flask. You can either program the feeding profile in the Drive manually using the wheel on top of the drive or wirelessly by using the LIS Software.
Do I have to clean the LIS Drive?
No, the drive is never in touch with the actual liquid but separated from it by a sterile filter. Thus, you can reuse it after every experiment. Since the drive contains sensitive electronics, you can not autoclave it.
What are the minimal and maximal dispensing volumes?
The minimal dispensing volume is a single drop. Depending on the type of liquid, concentration and temperature this volume can vary but is typically 50µl. The maximal dispensing volume is limited by the maximal flow rate of the drive, which is 1000µl per minute.
Which feeding profiles are available?
Currently, the LIS Drive is capable of dispensing single/multi shots and constant feeding. Additional feeding profiles (e.g. exponential feeding) and a feeding profile generator are available in our LIS Software.
What is the LIS Software?
The software, together with the coordinator, allows you to control and monitor your feeding experiments wirelessly. In the software you can create individual feeding profiles, archive experiments and individualize essential feeding parameters.
What is the LIS Coordinator?
The Coordinator is a transmitter box, which you can connect to your computer via USB. The LIS Software can control the LIS drives wirelessly through the Coordinator.
What is the LIS Cartridge?
The Cartridge is a single use container with the shape of a lid for shake flasks. During the experiment, the cartridge stores the liquid that you want to feed into your flask. It fits on all shake flasks that are normally sealed with a metal cap (straight neck shake flasks without a beaded rim and 38mm neck diameter). The cartridge comes sterile packed and is for one time use only.
How much liquid can be filled into the Cartridge?
Currently, our cartridges can be filled with up to 25ml of any liquid. If you want to feed more than 25ml, you can simply switch cartridges during the experiment and replace the empty one with a newly prepared one.
Can I reuse Cartridges?
No, the cartridge is a single use product. Sterility is not given after experimental usage. Additionally, the cartridge must be sealed air tight after filling it with liquid. aquila biolabs can only guarantee this proper fit (snap fit for air tightness between the cartridge and the lid of the cartridge) for first time usage.
How do I set up LIS?
If you have questions about the set up of LIS, contact us directly and we will gladly help you with the installation.
