The Liquid Injection System (LIS)
Do you want to perform feeding experiments in shake flasks?
Discover Liquid Injection System (LIS)
Wireless control and monitoring
Control and monitor your feeding experiments wirelessly with the LIS Software
Compatible with various substances
Sugars (e.g. 50 % glucose), alcohols (e.g. 75% methanol), glycerol (up to 75%), acids, bases, inductors (e.g. IPTG), antifoam and more
Create individual feeding profiles
Single/multi shot, constant/exponential feeding and many more
Easy to install and use
Fill the sterile cartridge, program the LIS drive and start feeding your culture
For a broad range of applications
Fed-batch, gene induction, pH regulation, phage assays, toxicity tests and more
What is the LIS Drive?
The Drive is a programmable miniature pump that controls when and how much liquid is fed from the cartridge into the flask. You can either program the feeding profile in the Drive manually using the wheel on top of the drive or wirelessly by using the LIS Software.
Do I have to clean the LIS Drive?
No, the drive is never in touch with the actual liquid but separated from it by a sterile filter. Thus, you can reuse it after every experiment. Since the drive contains sensitive electronics, you can not autoclave it.
What are the minimal and maximal dispensing volumes?
The minimal dispensing volume is a single drop. Depending on the type of liquid, concentration and temperature this volume can vary but is typically 50µl. The maximal dispensing volume is limited by the maximal flow rate of the drive, which is 1000µl per minute.
Under which shaking conditions can I use LIS?
The following matrix provides an overview on the maximal shaking speeds that can be used at 10 % filling volume of the flask together with LIS. Please note that these speeds are only valid for relatively new clamps and sticky stuff. Please test individually at which speed your flasks can be shaken safely together with LIS. If you intend to use LIS at shaking speeds higher than the ones listed, please contact us directly. We can supply special clamps that allow for high speed applications.
Which feeding profiles are available?
Currently the following profiles are available. Additional feeding profiles and a feeding profile generator are available in our software.
What is the LIS Software?
The software, together with the coordinator, allows you to control and monitor your feeding experiments wirelessly. In the software you can create individual feeding profiles, archive experiments and individualize essential feeding parameters.
What is the LIS Coordinator?
The Coordinator is a transmitter box, which you can connect to your computer via USB. The LIS Software can control the LIS drives wirelessly through the Coordinator.
What is the LIS Cartridge?
The Cartridge is a single use container with the shape of a lid for shake flasks. During the experiment, the cartridge stores the liquid that you want to feed into your flask. It fits on all shake flasks that are normally sealed with a metal cap (straight neck shake flasks without a beaded rim and 38mm neck diameter). The cartridge comes sterile packed and is for one time use only.
How much liquid can be filled into the Cartridge?
Currently, our cartridges can be filled with up to 25ml of any liquid. If you want to feed more than 25ml, you can simply switch cartridges during the experiment and replace the empty one with a newly prepared one.
Can I reuse Cartridges?
No, the cartridge is a single use product. Sterility is not given after experimental usage. Additionally, the cartridge must be sealed air tight after filling it with liquid. aquila biolabs can only guarantee this proper fit (snap fit for air tightness between the cartridge and the lid of the cartridge) for first time usage.
Which liquids can I fill into the Cartridge?
The table below provides an overview of successfully tested substances. If you are interested in a certain liquid and concentration that is not listed here, please contact us directly and we will inform or test for you if you can use your intended liquid.
How do I set up LIS?
In order to prepare and install LIS correctly, please take a look at our Quick Start Guide or contact us directly.